AAT Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0096R-Cy3-100uL |
| Product Name | AAT Polyclonal Antibody, Cy3 Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Cow, Pig |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human alpha 1 Antitrypsin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5265 |
| Subcellular location | Secreted, Extracellular matrix |
| Synonyms | Tryptase; Alpha-1-Antitrypsin; alpha 1 Antitrypsin; A1A; A1AT; AAT; Alpha 1 antiproteinase; Alpha 1 antitrypsin; Alpha 1 protease inhibitor; alpha-1-AT; alpha1 PI; alpha1 proteinase inhibitor; Clade A alpha 1 antiproteinase antitrypsin member 1; MGC23330; MGC9222; PI; PI; PRO2275; Protease inhibitor 1 anti elastase; Serpin A1; Serpin peptidase inhibitor clade A alpha 1 antiproteinase antitrypsin member 1; SerpinA1; A1AT_HUMAN; Alpha-1-antitrypsin; Alpha-1 protease inhibitor; Alpha-1-antiproteinase; Serpin A1. |
| Background | Alpha-1-antitrypsin is a protease inhibitor, deficiency of which is associated with emphysema and liver disease. The protein is encoded by a gene (PI) located on the distal long arm of chromosome 14. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is AAT Polyclonal Antibody, Cy3 Conjugated (bs-0096R-Cy3) validated for, and at what dilution?
bs-0096R-Cy3 is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does AAT Polyclonal Antibody, Cy3 Conjugated (bs-0096R-Cy3) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Pig.
How should bs-0096R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise AAT Polyclonal Antibody, Cy3 Conjugated (bs-0096R-Cy3)?
KLH conjugated synthetic peptide derived from human alpha 1 Antitrypsin
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-0096R-Cy3)?
Cy3 on bs-0096R-Cy3: excitation / emission 512,550nm/570,615nm.