PKM2 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0101R-Cy5.5-100uL |
| Product Name | PKM2 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse Pyruvate kinase PKM |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5315, 5313 |
| Swiss Prot | P14618, P30613 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-3; Pyruvate kinase PKM; Cytosolic thyroid hormone-binding protein; Opa-interacting protein 3; OIP-3; Pyruvate kinase 2/3; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; Tumor M2-PK; p58; PKM; PK2; Pyruvate kinase PKLR; Pyruvate kinase 1; R-type/L-type pyruvate kinase; Red cell/liver pyruvate kinase. |
| Background | Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is PKM2 Polyclonal Antibody, Cy5.5 Conjugated (BS-0101R-CY5-5) validated for, and at what dilution?
BS-0101R-CY5-5 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PKM2 Polyclonal Antibody, Cy5.5 Conjugated (BS-0101R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Pig, Horse, Rabbit.
How should BS-0101R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PKM2 Polyclonal Antibody, Cy5.5 Conjugated (BS-0101R-CY5-5)?
KLH conjugated synthetic peptide derived from mouse Pyruvate kinase PKM
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-0101R-CY5-5)?
Cy5.5 on BS-0101R-CY5-5: excitation / emission 675nm/694nm.