PKM2 Polyclonal Antibody, Cy7 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Cow
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-0101R-Cy7
Product Name PKM2 Polyclonal Antibody, Cy7 Conjugated
Applications WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Cow, Pig, Horse, Rabbit
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from mouse Pyruvate kinase PKM
Immunogen Range 255-350/531
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5315, 5313
Swiss Prot P14618, P30613
Subcellular location Cytoplasm, Nucleus
Synonyms PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-3; Pyruvate kinase PKM; Cytosolic thyroid hormone-binding protein; Opa-interacting protein 3; OIP-3; Pyruvate kinase 2/3; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; Tumor M2-PK; p58; PKM; PK2; Pyruvate kinase PKLR; Pyruvate kinase 1; R-type/L-type pyruvate kinase; Red cell/liver pyruvate kinase.
Background Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200