Leptin Receptor Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0109R-BF488 |
| Product Name | Leptin Receptor Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This antibody will recognize the Isoform B variant only |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Sheep, Pig |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Leptin receptor:901-1000/1165 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3953 |
| Swiss Prot | P48357 |
| Subcellular location | Secreted, Cell membrane |
| Synonyms | OBR; OB-R; CD295; LEP-R; LEPRD; Leptin receptor; HuB219; OB receptor; LEPR; DB; LEPR_MOUSE |
| Background | Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Leptin Receptor Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-0109R-BF488) validated for, and at what dilution?
bs-0109R-BF488 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Leptin Receptor Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-0109R-BF488) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig.
How should bs-0109R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Leptin Receptor Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-0109R-BF488)?
KLH conjugated synthetic peptide derived from human Leptin receptor:901-1000/1165
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-0109R-BF488)?
AbBy Fluor-488 on bs-0109R-BF488: excitation / emission 499nm/519nm.