AKT1/2/3 Polyclonal Antibody, Biotin Conjugated

Applications

  • WB
  • IHC-P
  • IHC-F

Reactivity

  • Human
  • Mouse
  • Rat
  • Bovine
  • Pig
  • Others

Predicted Reactivity

  • Dog
  • Cow
  • Sheep
  • Chicken
Overview
Catalog # bs-0115R-Biotin
Product Name AKT1/2/3 Polyclonal Antibody, Biotin Conjugated
Applications WB, IHC-P, IHC-F
Reactivity Human, Mouse, Rat, Bovine, Pig, Others
Predicted Reactivity Dog, Cow, Sheep, Chicken
Specifications
Conjugation Biotin
Host Rabbit
Source KLH conjugated synthetic peptide derived from human AKT1
Immunogen Range 401-479/479
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 207
Swiss Prot P31749
Subcellular location Cytoplasm, Nucleus, Cell membrane
Synonyms AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; Protein kinase B; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT1; RAC-gamma serine/threonine-protein kinase; Protein kinase Akt-3; Protein kinase B gamma; PKB gamma; RAC-PK-gamma; STK-2; PKBG
Background AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility; prohibitin (PHB), playing an important role in cell metabolism and proliferation; and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500