Estrogen receptor beta Antibody, PE Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0116R-PE-100uL |
| Product Name | Estrogen receptor beta Antibody, PE Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ER-beta |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2100 |
| Swiss Prot | Q92731 |
| Subcellular location | Nucleus |
| Synonyms | Erb; ESRB; ESTRB; NR3A2; ER-BETA; ESR-BETA; Estrogen receptor beta; Nuclear receptor subfamily 3 group A member 2; ESR2 |
| Background | Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | FCM1:20-100 |
Frequently asked questions
Which applications is Estrogen receptor beta Antibody, PE Conjugated (bs-0116R-PE) validated for, and at what dilution?
bs-0116R-PE is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Estrogen receptor beta Antibody, PE Conjugated (bs-0116R-PE) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-0116R-PE be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Estrogen receptor beta Antibody, PE Conjugated (bs-0116R-PE)?
KLH conjugated synthetic peptide derived from human ER-beta
What are the excitation and emission wavelengths of the PE conjugate (bs-0116R-PE)?
PE on bs-0116R-PE: excitation / emission 496,564nm/578nm.