HSP70 Polyclonal Antibody, PE Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0126R-PE |
| Product Name | HSP70 Polyclonal Antibody, PE Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Cow, Sheep, Chicken, Rabbit |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HSP70 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3303 |
| Swiss Prot | P08107 |
| Subcellular location | Cytoplasm |
| Synonyms | HSP72; HSPA1; HSP7I; HSP7-1; HSP7-1A; HEL-S-13; Heat shock 7 kDa protein 1A/1B; Heat shock 7 kDa protein 1/2; HSP7-1/HSP7-2; HSP7.1/HSP7.2; HSPA1A; HSX7 |
| Background | In cooperation with other chaperones, Hsp7s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | |
| IF(IHC-F) | |
| IF(ICC) | |
Frequently asked questions
Which applications is HSP70 Polyclonal Antibody, PE Conjugated (bs-0126R-PE) validated for, and at what dilution?
bs-0126R-PE is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HSP70 Polyclonal Antibody, PE Conjugated (bs-0126R-PE) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep, Chicken, Rabbit.
How should bs-0126R-PE be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HSP70 Polyclonal Antibody, PE Conjugated (bs-0126R-PE)?
KLH conjugated synthetic peptide derived from human HSP70
What are the excitation and emission wavelengths of the PE conjugate (bs-0126R-PE)?
PE on bs-0126R-PE: excitation / emission 496,564nm/578nm.