Nogo-A Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0134r-cy55-100uL |
| Product Name | Nogo-A Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse RTN4 protein |
| Immunogen Range | 301-400/1192 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 68585 |
| Swiss Prot | Q99P72 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | ASY; NgA; NOGO; NSP-CL; Nogo-A; Nogo-B; Nogo-C; AA407876; AA409940; AA960376; mKIAA0886; mKIAA4153; 1110020G17Rik; C130026I10Rik; Reticulon-4; Neurite outgrowth inhibitor; Nogo protein; Rtn4; Kiaa0886 |
| Background | Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex. May inhibit BACE1 activity and amyloid precursor protein processing. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Nogo-A Polyclonal Antibody, Cy5.5 Conjugated (BS-0134R-CY5-5) validated for, and at what dilution?
BS-0134R-CY5-5 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Nogo-A Polyclonal Antibody, Cy5.5 Conjugated (BS-0134R-CY5-5) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse.
How should BS-0134R-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Nogo-A Polyclonal Antibody, Cy5.5 Conjugated (BS-0134R-CY5-5)?
KLH conjugated synthetic peptide derived from mouse RTN4 protein Immunogen range: 301-400/1192.
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-0134R-CY5-5)?
Cy5.5 on BS-0134R-CY5-5: excitation / emission 675nm/694nm.