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TRA1 variant Polyclonal Antibody, Cy5.5 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Pig
  • Horse
  • Chicken
  • Rabbit
  • GuineaPig
Overview
Catalog # bs-0149R-Cy5.5-100uL
Product Name TRA1 variant Polyclonal Antibody, Cy5.5 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Pig, Horse, Chicken, Rabbit, GuineaPig
Specifications
Conjugation Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human TRA1 variant
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Subcellular location Cytoplasm
Synonyms tumor rejection antigen (gp96) 1 variant; TRA1.
Background TRA1 variant
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is TRA1 variant Polyclonal Antibody, Cy5.5 Conjugated (BS-0149R-CY5-5) validated for, and at what dilution?

BS-0149R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TRA1 variant Polyclonal Antibody, Cy5.5 Conjugated (BS-0149R-CY5-5) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Pig, Horse, Chicken, Rabbit, GuineaPig.

How should BS-0149R-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise TRA1 variant Polyclonal Antibody, Cy5.5 Conjugated (BS-0149R-CY5-5)?

KLH conjugated synthetic peptide derived from human TRA1 variant

What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-0149R-CY5-5)?

Cy5.5 on BS-0149R-CY5-5: excitation / emission 675nm/694nm.