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Tubulin-alpha Polyclonal Antibody

Applications

  • WB
  • ELISA
  • FCM
  • IHC-P
  • IF(IHC-P)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Cow
  • Sheep
Overview
Catalog # bs-0159r-100uL
Product Name Tubulin-alpha Polyclonal Antibody
Applications
WB ELISA FCM IHC-P IF(IHC-P)
Specificity will recognize many forms of alpha tubulin, Tubulin alpha-1A chain, Tubulin alpha-4A chain etc
Reactivity Human, Mouse, Rat
Predicted Reactivity Cow, Sheep
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Tubulin-alpha 1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 7277
Swiss Prot P68366
Subcellular location Cytoplasm
Synonyms TUBA1; H2-ALPHA; Tubulin alpha-4A chain; Alpha-tubulin 1; Testis-specific alpha-tubulin; Tubulin H2-alpha; Tubulin alpha-1 chain; TUBA4A
Background Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Frequently asked questions

Which applications is Tubulin-alpha Polyclonal Antibody (bs-0159R) validated for, and at what dilution?

bs-0159R is validated for WB (1:300-5000); ELISA (1:500-1000); FCM (1:20-100); IHC-P (1:200-400); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Tubulin-alpha Polyclonal Antibody (bs-0159R) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep.

How should bs-0159R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Tubulin-alpha Polyclonal Antibody (bs-0159R)?

KLH conjugated synthetic peptide derived from human Tubulin-alpha 1

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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