Tubulin-alpha Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0159R-BF647 |
| Product Name | Tubulin-alpha Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
|
| Specificity | will recognize many forms of alpha tubulin, Tubulin alpha-1A chain, Tubulin alpha-4A chain etc |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Cow, Sheep |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Tubulin-alpha 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7277 |
| Swiss Prot | P68366 |
| Subcellular location | Cytoplasm |
| Synonyms | TUBA1; H2-ALPHA; Tubulin alpha-4A chain; Alpha-tubulin 1; Testis-specific alpha-tubulin; Tubulin H2-alpha; Tubulin alpha-1 chain; TUBA4A |
| Background | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is Tubulin-alpha Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-0159R-BF647) validated for, and at what dilution?
bs-0159R-BF647 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Tubulin-alpha Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-0159R-BF647) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep.
How should bs-0159R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Tubulin-alpha Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-0159R-BF647)?
KLH conjugated synthetic peptide derived from human Tubulin-alpha 1
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-0159R-BF647)?
AbBy Fluor-647 on bs-0159R-BF647: excitation / emission 650nm/665nm.