IRS1 Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0172r-100uL |
| Product Name | IRS1 Polyclonal Antibody |
| Applications |
IHC-P
IHC-F
IF
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Cow, Sheep, Horse, Rabbit |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human IRS-1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 3667 |
| Synonyms | HIRS 1; HIRS1; Insulin Receptor Substrate 1; IRS-1; IRS 1; IRS1; OTTHUMP00000164234; IRS1_HUMAN. |
| Background | Insulin receptor substrates (IRS) are responsible for several insulin related activities, such as glucose homeostasis, cell growth, cell transformation, apoptosis and insulin signal transduction. Serine/threonine phosphorylation of IRS1 has been demonstrated to be a negative regulator of insulin signaling and is responsible for its degradation, although IRS1 degradation pathways are not well understood. IRS1 has also been shown to be constitutively activated in cancers such as breast cancer, Wilm's tumors, and adrenal cortical carcinomas, thus making IRS1 phosphorylation and subsequent degradation an attractive therapeutic target. To date there have been four subtypes identified: IRS1, 2, 3 and 4, with IRS1 being widely expressed. |
| Application Dilution | |
| IHC-P | 1:100-500 |
| IHC-F | 1:100-500 |
| IF | 1:100-500 |
Frequently asked questions
Which applications is IRS1 Polyclonal Antibody (bs-0172R) validated for, and at what dilution?
bs-0172R is validated for IHC-P (1:100-500); IHC-F (1:100-500); IF (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does IRS1 Polyclonal Antibody (bs-0172R) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Cow, Sheep, Horse, Rabbit.
How should bs-0172R be stored?
Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise IRS1 Polyclonal Antibody (bs-0172R)?
KLH conjugated synthetic peptide derived from human IRS-1