RAGE Polyclonal Antibody, PE-Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0177r-pe-cy55-100uL |
| Product Name | RAGE Polyclonal Antibody, PE-Cy5.5 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from rat AGER |
| Immunogen Range | 151-250/403 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 81722 |
| Swiss Prot | Q63495 |
| Subcellular location | Secreted, Cell membrane |
| Synonyms | RAGE; Advanced glycosylation end product-specific receptor; Receptor for advanced glycosylation end products; Ager |
| Background | Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S1A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Can also bind oligonucleotides (By similarity). Interaction with S1B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is RAGE Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-0177R-PE-CY5-5) validated for, and at what dilution?
BS-0177R-PE-CY5-5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RAGE Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-0177R-PE-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BS-0177R-PE-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RAGE Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-0177R-PE-CY5-5)?
KLH conjugated synthetic peptide derived from rat AGER Immunogen range: 151-250/403.
What are the excitation and emission wavelengths of the PE-Cy5.5 conjugate (BS-0177R-PE-CY5-5)?
PE-Cy5.5 on BS-0177R-PE-CY5-5: excitation / emission 488nm/694nm.