Nociceptin receptor Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0181R-BF680 |
| Product Name | Nociceptin receptor Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Pig, Horse, GuineaPig |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse Nociceptin receptor |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 18389 |
| Swiss Prot | P35377 |
| Subcellular location | Cell membrane |
| Synonyms | KOR3; ORGC; ORL1; Oprl; XOR1; morc; KOR-3; LC132; MOR-C; Nociceptin receptor; K3 opiate receptor; Kappa-type 3 opioid receptor; Orphanin FQ receptor; Oprl1; Oor |
| Background | G-protein coupled opioid receptor that functions as receptor for the endogenous neuropeptide nociceptin. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling via G proteins mediates inhibition of adenylate cyclase activity and calcium channel activity. Arrestins modulate signaling via G proteins and mediate the activation of alternative signaling pathways that lead to the activation of MAP kinases. Plays a role in modulating nociception and the perception of pain. Plays a role in the regulation of locomotor activity by the neuropeptide nociceptin. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Nociceptin receptor Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-0181R-BF680) validated for, and at what dilution?
bs-0181R-BF680 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Nociceptin receptor Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-0181R-BF680) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Horse, GuineaPig.
How should bs-0181R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Nociceptin receptor Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-0181R-BF680)?
KLH conjugated synthetic peptide derived from mouse Nociceptin receptor
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-0181R-BF680)?
AbBy Fluor-680 on bs-0181R-BF680: excitation / emission 679nm/702nm.