IRS3 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0186R-Cy3-100uL |
| Product Name | IRS3 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat, Pig |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from rat IRS-3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 84021 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | IRS1--Insulin receptor substrate-3; IRS3; IRS-3; IRS3. |
| Background | The family of insulin receptor substrates (IRSs) has been reported to play important roles for signal transduction of various hormones. Four members of the IRS family have been described. Each IRS is believed to have different functions; however, the distinct physiological roles of each IRS are unclear. IRS-1 may mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is IRS3 Polyclonal Antibody, Cy3 Conjugated (bs-0186R-Cy3) validated for, and at what dilution?
bs-0186R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does IRS3 Polyclonal Antibody, Cy3 Conjugated (bs-0186R-Cy3) react with?
Tested reactivity: Mouse, Rat, Pig.
How should bs-0186R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise IRS3 Polyclonal Antibody, Cy3 Conjugated (bs-0186R-Cy3)?
KLH conjugated synthetic peptide derived from rat IRS-3
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-0186R-Cy3)?
Cy3 on bs-0186R-Cy3: excitation / emission 512,550nm/570,615nm.