IRS-4 Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0187R-BF488 |
| Product Name | IRS-4 Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Mouse |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse IRS-4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 16370 |
| Swiss Prot | Q9Z0Y7 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Insulin receptor substrate 4; IRS-4; Phosphoprotein of 160 kDa; pp160 |
| Background | Acts as an interface between multiple growth factor receptors possessing tyrosine kinase activity, such as insulin receptor, IGF1R and FGFR1, and a complex network of intracellular signaling molecules containing SH2 domains. Involved in the IGF1R mitogenic signaling pathway. Promotes the AKT1 signaling pathway and BAD phosphorylation during insulin stimulation without activation of RPS6KB1 or the inhibition of apoptosis. Interaction with GRB2 enhances insulin-stimulated mitogen-activated protein kinase activity. May be involved in nonreceptor tyrosine kinase signaling in myoblasts. Plays a pivotal role in the proliferation/differentiation of hepatoblastoma cell through EPHB2 activation upon IGF1 stimulation. May play a role in the signal transduction in response to insulin and to a lesser extent in response to IL4 and GH on mitogenesis. Plays a role in growth, reproduction and glucose homeostasis. May act as negative regulators of the IGF1 signaling pathway by suppressing the function of IRS1 and IRS2. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is IRS-4 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-0187R-BF488) validated for, and at what dilution?
bs-0187R-BF488 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does IRS-4 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-0187R-BF488) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse.
How should bs-0187R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise IRS-4 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-0187R-BF488)?
KLH conjugated synthetic peptide derived from mouse IRS-4
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-0187R-BF488)?
AbBy Fluor-488 on bs-0187R-BF488: excitation / emission 499nm/519nm.