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GDNFRA Polyclonal Antibody, APC Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Pig
  • Horse
Overview
Catalog # bs-0201R-APC
Product Name GDNFRA Polyclonal Antibody, APC Conjugated
Applications
WB FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Specificity This antibody exhibits a secondary cross-reactivity towards Exonuclease-1 due to a 60% contigueous sequence similarity over the immunogen range.
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Pig, Horse
Specifications
Conjugation APC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human GFR Alpha-1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2674
Swiss Prot P56159
Subcellular location Cell membrane
Synonyms GDNFR; RET1L; RETL1; TRNR1; GDNFRA; GFR-ALPHA-1; GDNF family receptor alpha-1; GDNF receptor alpha-1; GDNFR-alpha-1; RET ligand 1; TGF-beta-related neurotrophic factor receptor 1; GFRA1
Background Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor (By similarity).
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is GDNFRA Polyclonal Antibody, APC Conjugated (bs-0201R-APC) validated for, and at what dilution?

bs-0201R-APC is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does GDNFRA Polyclonal Antibody, APC Conjugated (bs-0201R-APC) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Horse.

How should bs-0201R-APC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise GDNFRA Polyclonal Antibody, APC Conjugated (bs-0201R-APC)?

KLH conjugated synthetic peptide derived from human GFR Alpha-1

What are the excitation and emission wavelengths of the APC conjugate (bs-0201R-APC)?

APC on bs-0201R-APC: excitation / emission 650nm/660nm.