PPET1 Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0202r-bf680 |
| Product Name | PPET1 Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Big endothelin-1 |
| Immunogen Range | 81-212/212 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1906 |
| Swiss Prot | P05305 |
| Subcellular location | Secreted |
| Synonyms | EDN1_HUMAN; Big endothelin 1; EDN 1; EDN1; EDN1 protein; Endothelin1; Endothelin 1; Endothelin-1; ET 1; ET1; OTTHUMP00000039245; PPET1; |
| Background | Big Endothelin-1 is precursor of endothelin 1. Big endothelin-1 is cleaved to yield endothelin-1 via the activity of an endothelin-converting enzyme (ECE). Big Endothelin-1 can be hydrolyzed by chymase to generate endothelin 1 (1-21) in vitro. Endothelins are endothelium-derived vasoconstrictor peptides. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is PPET1 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-0202R-BF680) validated for, and at what dilution?
bs-0202R-BF680 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PPET1 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-0202R-BF680) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-0202R-BF680 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PPET1 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-0202R-BF680)?
KLH conjugated synthetic peptide derived from human Big endothelin-1 Immunogen range: 81-212/212.
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-0202R-BF680)?
AbBy Fluor-680 on bs-0202R-BF680: excitation / emission 679nm/702nm.