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TSHR Polyclonal Antibody, Cy5.5 Conjugated

Applications

  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Rat
  • Cow
  • Pig
  • Horse
  • Chicken
  • Rabbit
  • GuineaPig
Overview
Catalog # bs-0206R-Cy5.5-100uL
Product Name TSHR Polyclonal Antibody, Cy5.5 Conjugated
Applications
FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Rat, Cow, Pig, Horse, Chicken, Rabbit, GuineaPig
Specifications
Conjugation Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human TSHR
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 7253
Swiss Prot P16473
Subcellular location Cell membrane
Synonyms LGR3; CHNG1; hTSHR-I; Thyrotropin receptor; Thyroid-stimulating hormone receptor; TSH-R; TSHR
Background Receptor for thyrothropin. Plays a central role in controlling thyroid cell metabolism. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Also acts as a receptor for thyrostimulin (GPA2+GPB5).
Application Dilution
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is TSHR Polyclonal Antibody, Cy5.5 Conjugated (BS-0206R-CY5-5) validated for, and at what dilution?

BS-0206R-CY5-5 is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TSHR Polyclonal Antibody, Cy5.5 Conjugated (BS-0206R-CY5-5) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Cow, Pig, Horse, Chicken, Rabbit, GuineaPig.

How should BS-0206R-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise TSHR Polyclonal Antibody, Cy5.5 Conjugated (BS-0206R-CY5-5)?

KLH conjugated synthetic peptide derived from human TSHR

What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-0206R-CY5-5)?

Cy5.5 on BS-0206R-CY5-5: excitation / emission 675nm/694nm.

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