CD133 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0209R-Cy5.5 |
| Product Name | CD133 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
FCM
IF
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Fruit Fly |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CD133 antigen |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8842 |
| Swiss Prot | O43490 |
| Subcellular location | Cell membrane |
| Synonyms | AC133; Antigen AC133; Hematopoietic stem cell antigen; hProminin; PROM1; Prominin I; Prominin like protein 1 precursor; Prominin mouse like 1; prominin1; PROML1; CD133; CORD12; MCDR2; MSTP061; PROML1; RP41; STGD4; PROM1_HUMAN. |
| Background | Binds cholesterol in cholesterol-containing plasma membrane microdomains. Proposed to play a role in apical plasma membrane organization of epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF | |
Frequently asked questions
Which applications is CD133 Polyclonal Antibody, Cy5.5 Conjugated (BS-0209R-CY5-5) validated for, and at what dilution?
BS-0209R-CY5-5 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD133 Polyclonal Antibody, Cy5.5 Conjugated (BS-0209R-CY5-5) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Fruit Fly.
How should BS-0209R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD133 Polyclonal Antibody, Cy5.5 Conjugated (BS-0209R-CY5-5)?
KLH conjugated synthetic peptide derived from human CD133 antigen
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-0209R-CY5-5)?
Cy5.5 on BS-0209R-CY5-5: excitation / emission 675nm/694nm.