HCV-NS4a Polyclonal Antibody

Applications

  • ELISA

Reactivity

  • Virus
Overview
Catalog # bs-0213R
Product Name HCV-NS4a Polyclonal Antibody
Applications ELISA
Reactivity Virus
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from HCV-NS4a
Immunogen Range 1658-1711/3010
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Subcellular location Cytoplasm, Cell membrane
Synonyms HCV-NS4a; Non-structural protein 4A; NS4A;POLG_HCVBK.
Background Hepatitis C virus (HCV) is an enveloped, positive-strand RNA virus classified within the genus Hepacivirus in the family Flaviviridae. The nonsegmented HCV genome is 9.6-kb and encodes a long polyprotein that is cleaved by cellular and viral proteases into at least 10 distinct products. The N-terminal region of this polyprotein encodes the viral structural proteins: core and two envelope glycoproteins, E1 and E2. The remainder of the polyprotein encodes nonstructural (NS) proteins: p7, NS2, NS3, NS4A, NS4B, NS5A, and NS5B. NS4A is the smallest NS protein. It encodes an N-terminal hydrophobic alpha-helix that anchors NS3-4A to cellular membranes, a middle cofactor peptide that forms a beta-strand and contributes to proper folding of the NS3 serine protease domain, and a C-terminal acidic region that forms an alpha-helix at low pH.
Application Dilution
ELISA 1:500-1000