DFFA Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0214R-BF750-100uL |
| Product Name | DFFA Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse ICAD |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 13347 |
| Swiss Prot | O54786 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | ICAD; DFF35; Dff45; ICAD-L; ICAD-S; A330085O09Rik; DNA fragmentation factor subunit alpha; DNA fragmentation factor 45 kDa subunit; DFF-45; Inhibitor of CAD; Dffa |
| Background | Inhibitor of the caspase-activated DNase (DFF40). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DFFA Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-0214R-BF750) validated for, and at what dilution?
bs-0214R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DFFA Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-0214R-BF750) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-0214R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DFFA Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-0214R-BF750)?
KLH conjugated synthetic peptide derived from mouse ICAD
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-0214R-BF750)?
AbBy Fluor-750 on bs-0214R-BF750: excitation / emission 749nm/775nm.