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FGF1 Polyclonal Antibody

Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti FGF1\/AFGF Polyclonal Antibody, Unconjugated (bs-0229R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti FGF1\/AFGF Polyclonal Antibody, Unconjugated (bs-0229R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti FGF1\/AFGF Polyclonal Antibody, Unconjugated (bs-0229R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti FGF1\/AFGF Polyclonal Antibody, Unconjugated (bs-0229R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse heart lysates probed with Rabbit Anti-FGF1 Polyclonal Antibody, Unconjugated (bs-0229R) at 1:300 overnight at 4\u02daC. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
Mouse heart lysates probed with Rabbit Anti-FGF1 Polyclonal Antibody, Unconjugated (bs-0229R) at 1:300 overnight at 4\u02daC. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
A431 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with FGF1 Polyclonal Antibody(bs-0229R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
A431 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with FGF1 Polyclonal Antibody(bs-0229R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Bioss unconjugated antibody vial

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Sheep
Overview
Catalog # bs-0229R
Product Name FGF1 Polyclonal Antibody
Applications
WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse
Predicted Reactivity Rat, Sheep
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from mouse FGF1
Immunogen Range 16-80/155
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 14164
Swiss Prot P61148
Subcellular location Cytoplasm, Nucleus, Secreted, Cell membrane
Synonyms Fam; Fgfa; Dffrx; Fgf-1; Fibroblast growth factor 1; Acidic fibroblast growth factor; aFGF; Heparin-binding growth factor 1; HBGF-1; Fgf1
Background Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro (By similarity).
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is FGF1 Polyclonal Antibody (bs-0229R) validated for, and at what dilution?

bs-0229R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does FGF1 Polyclonal Antibody (bs-0229R) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Sheep.

How should bs-0229R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise FGF1 Polyclonal Antibody (bs-0229R)?

KLH conjugated synthetic peptide derived from mouse FGF1 Immunogen range: 16-80/155.

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