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FGF1 Polyclonal Antibody, AbBy Fluor-750 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Sheep
Overview
Catalog # bs-0229R-BF750-100uL
Product Name FGF1 Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse
Predicted Reactivity Rat, Sheep
Specifications
Conjugation AbBy Fluor-750
Host Rabbit
Source KLH conjugated synthetic peptide derived from mouse FGF1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 14164
Swiss Prot P61148
Subcellular location Cytoplasm, Nucleus, Secreted, Cell membrane
Synonyms Fam; Fgfa; Dffrx; Fgf-1; Fibroblast growth factor 1; Acidic fibroblast growth factor; aFGF; Heparin-binding growth factor 1; HBGF-1; Fgf1
Background Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro (By similarity).
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is FGF1 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-0229R-BF750) validated for, and at what dilution?

bs-0229R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does FGF1 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-0229R-BF750) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Sheep.

How should bs-0229R-BF750 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise FGF1 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-0229R-BF750)?

KLH conjugated synthetic peptide derived from mouse FGF1

What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-0229R-BF750)?

AbBy Fluor-750 on bs-0229R-BF750: excitation / emission 749nm/775nm.