EXPB-2 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Tomato leaf; Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with EXPB-2 Polyclonal Antibody, Unconjugated (bs-0243R) at 1:400 overnight at 4\u2103, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Tomato leaf; Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with EXPB-2 Polyclonal Antibody, Unconjugated (bs-0243R) at 1:400 overnight at 4\u2103, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Plant

Overview
Catalog #	bs-0243R
Product Name	EXPB-2 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Plant
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from Triticum aestivum EXPB-2
Immunogen Range	10-110/265
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot	Q6QN86
Synonyms	beta-expansin 2; expansin EXPB5; Q6QN86_WHEAT.
Background	beta-expansin (EXBP2) may cause loosening and extension of plant cell walls by disrupting noncovalent bonding between cellulose microfibrils and matrix glucans. No enzymatic activity has been found Subcellular Location at Cell-wall bound. Belongs to the expansin family.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200

Applications

Reactivity

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