HSP70 Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0244R-100uL |
| Product Name | HSP70 Polyclonal Antibody |
| Applications |
WB
IHC-P
IHC-F
IF
FLOW-CYT
ICC/IF
|
| Reactivity | Human, Mouse, Rat, Pig, Chicken |
| Predicted Reactivity | Cow, Sheep, Rabbit |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HSP70 1A/1B |
| Immunogen Range | 500-600/641 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 3303 |
| Swiss Prot | P08107 |
| Subcellular location | Cytoplasm |
| Synonyms | HSP72; HSPA1; HSP70I; HSP70-1; HSP70-1A; HEL-S-103; Heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1/2; HSP70-1/HSP70-2; HSP70.1/HSP70.2; HSPA1A; HSX70 |
| Background | In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | ELISA1:500-1000 |
| IHC-F | FCM1:20-100 |
| IF | IHC-P1:200-400 |
| Flow-Cyt | |
| ICC/IF | |
Frequently asked questions
Which applications is HSP70 Polyclonal Antibody (bs-0244R) validated for, and at what dilution?
bs-0244R is validated for WB (1:300-5000); ELISA (1:500-1000); FCM (1:20-100); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HSP70 Polyclonal Antibody (bs-0244R) react with?
Tested reactivity: Human, Mouse, Rat, Pig, Chicken. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep, Rabbit.
How should bs-0244R be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HSP70 Polyclonal Antibody (bs-0244R)?
KLH conjugated synthetic peptide derived from human HSP70 1A/1B Immunogen range: 500-600/641.