EphB2 Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0247R-BF680 |
| Product Name | EphB2 Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human EphB2 R |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2048 |
| Swiss Prot | P29323 |
| Subcellular location | Cell membrane |
| Synonyms | DRT; EK5; ERK; CAPB; Hek5; PCBC; EPHT3; Tyro5; Ephrin type-B receptor 2; Developmentally-regulated Eph-related tyrosine kinase; ELK-related tyrosine kinase; EPH tyrosine kinase 3; EPH-like kinase 5; Renal carcinoma antigen NY-REN-47; Tyrosine-protein kinase TYRO5; Tyrosine-protein kinase receptor EPH-3; EPHB2; EPTH3 |
| Background | Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Functions in axon guidance during development. Involved in the guidance of commissural axons, that form a major interhemispheric connection between the 2 temporal lobes of the cerebral cortex. Also involved in guidance of contralateral inner ear efferent growth cones at the midline and of retinal ganglion cell axons to the optic disk. In addition to axon guidance, also regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses. Upon activation by EFNB1, abolishes the ARHGEF15-mediated negative regulation on excitatory synapse formation. Controls other aspects of development including angiogenesis, palate development and in inner ear development through regulation of endolymph production. Forward and reverse signaling through the EFNB2/EPHB2 complex regulate movement and adhesion of cells that tubularize the urethra and septate the cloaca. May function as a tumor suppressor. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is EphB2 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-0247R-BF680) validated for, and at what dilution?
bs-0247R-BF680 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does EphB2 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-0247R-BF680) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Chicken.
How should bs-0247R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise EphB2 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-0247R-BF680)?
KLH conjugated synthetic peptide derived from human EphB2 R
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-0247R-BF680)?
AbBy Fluor-680 on bs-0247R-BF680: excitation / emission 679nm/702nm.