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PPAR delta + beta Polyclonal Antibody, Biotin Conjugated

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
Overview
Catalog # bs-0250R-Biotin-100uL
Product Name PPAR delta + beta Polyclonal Antibody, Biotin Conjugated
Applications
WB ELISA IHC-P IHC-F
Reactivity Human, Mouse
Predicted Reactivity Rat
Specifications
Conjugation Biotin
Host Rabbit
Source KLH conjugated synthetic peptide derived from mouse PPAR-delta
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C for 12 months.
Target
Gene ID 19015
Swiss Prot P35396
Subcellular location Nucleus
Synonyms NUC1; NUC-1; Nr1c2; Pparb; PPAR[b]; Pparb/d; PPAR-beta; PPARdelta; PPAR-delta; Peroxisome proliferator-activated receptor delta; Nuclear hormone receptor 1; Nuclear receptor subfamily 1 group C member 2; Peroxisome proliferator-activated receptor beta; Ppard
Background Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand (By similarity).
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is PPAR delta + beta Polyclonal Antibody, Biotin Conjugated (bs-0250R-Biotin) validated for, and at what dilution?

bs-0250R-Biotin is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PPAR delta + beta Polyclonal Antibody, Biotin Conjugated (bs-0250R-Biotin) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.

How should bs-0250R-Biotin be stored?

Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise PPAR delta + beta Polyclonal Antibody, Biotin Conjugated (bs-0250R-Biotin)?

KLH conjugated synthetic peptide derived from mouse PPAR-delta

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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