RAR alpha Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0251r-bf405-100uL |
| Product Name | RAR alpha Polyclonal Antibody, AbBy Fluor-405 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | OVA conjugated synthetic peptide derived from mouse Retinoic acid Receptor Alpha |
| Immunogen Range | 361-462/462 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 19401 |
| Swiss Prot | P11416 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | RAR; Nr1b1; RARalpha1; Retinoic acid receptor alpha; RAR-alpha; Nuclear receptor subfamily 1 group B member 1; Rara |
| Background | Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. Regulates expression of target genes in a ligand-dependent manner by recruiting chromatin complexes containing KMT2E/MLL5. Mediates retinoic acid-induced granulopoiesis. RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Has a role in the survival of early spermatocytes at the beginning prophase of meiosis. In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is RAR alpha Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-0251R-BF405) validated for, and at what dilution?
bs-0251R-BF405 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RAR alpha Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-0251R-BF405) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.
How should bs-0251R-BF405 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RAR alpha Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-0251R-BF405)?
OVA conjugated synthetic peptide derived from mouse Retinoic acid Receptor Alpha Immunogen range: 361-462/462.
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-0251R-BF405)?
AbBy Fluor-405 on bs-0251R-BF405: excitation / emission 401nm/421nm.