MAG Polyclonal Antibody

Name: MAG Polyclonal Antibody
Brand: Bioss
SKU: bs-0258R
Price: 375.0 USD
Availability: InStock

$Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-MAG-a\/L-MAG Polyclonal Antibody (bs-0258R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-MAG-a\/L-MAG Polyclonal Antibody (bs-0258R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

$Lane 1: Rat Cerebrum tissue lysates; Lane 2: Rat Cerebellum tissue lysates; Lane 3: Rat Spinal cord tissue lysates probed with MAG Polyclonal Antibody, Unconjugated (bs-0258R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Rat Cerebrum tissue lysates; Lane 2: Rat Cerebellum tissue lysates; Lane 3: Rat Spinal cord tissue lysates probed with MAG Polyclonal Antibody, Unconjugated (bs-0258R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

$25 ug total protein per lane of various lysates (see on figure) probed with MAG polyclonal antibody, unconjugated (bs-0258R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with MAG polyclonal antibody, unconjugated (bs-0258R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MAG Polyclonal Antibody, Unconjugated (bs-0258R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MAG Polyclonal Antibody, Unconjugated (bs-0258R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MAG Polyclonal Antibody, Unconjugated (bs-0258R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MAG Polyclonal Antibody, Unconjugated (bs-0258R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MAG Polyclonal Antibody, Unconjugated (bs-0258R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MAG Polyclonal Antibody, Unconjugated (bs-0258R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MAG Polyclonal Antibody, Unconjugated (bs-0258R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MAG Polyclonal Antibody, Unconjugated (bs-0258R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MAG Polyclonal Antibody, Unconjugated (bs-0258R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MAG Polyclonal Antibody, Unconjugated (bs-0258R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MAG Polyclonal Antibody, Unconjugated (bs-0258R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MAG Polyclonal Antibody, Unconjugated (bs-0258R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Applications

WB
ELISA
IHC-F
IF
IHC-P
Flow-Cyt

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-0258r-100uL
Product Name	MAG Polyclonal Antibody
Applications	WB ELISA IHC-F IF IHC-P FLOW-CYT
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human MAG
Immunogen Range	551-626/626
Clonality	Polyclonal
Isotype	IgG
Concentration	Lot Dependent
Purification	purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4099
Swiss Prot	P20916
Subcellular location	Cell membrane
Synonyms	GMA; S-MAG; SIGLEC4A; SIGLEC-4A; Myelin-associated glycoprotein; MAG
Background	Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity).
Application Dilution
WB	1:500-2000
ELISA	1:5000-10000
IHC-F	1:50-200
IF	1:50-200
IHC-P	1:50-200
Flow-Cyt	1:50-100

Frequently asked questions

Which applications is MAG Polyclonal Antibody (bs-0258R) validated for, and at what dilution?

bs-0258R is validated for WB (1:500-2000); ELISA (1:5000-10000); IHC-F (1:50-200); IF (1:50-200); IHC-P (1:50-200); Flow-Cyt (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does MAG Polyclonal Antibody (bs-0258R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-0258R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise MAG Polyclonal Antibody (bs-0258R)?

KLH conjugated synthetic peptide derived from human MAG Immunogen range: 551-626/626.

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MAG Polyclonal Antibody

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Reactivity

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