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AQP2 Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-0261R-Cy3-100uL
Product Name AQP2 Polyclonal Antibody, Cy3 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human AQP2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 84062
Swiss Prot P41181
Subcellular location Cytoplasm, Cell membrane
Synonyms ADH water channel; AQP 2; AQP-2; AQP CD; AQP2; AQPCD; Aquaporin 2 collecting duct; Aquaporin CD; Aquaporin2; Aquaporine 2; Collecting duct water channel protein; MGC34501; Water channel protein for renal collecting duct; WCH CD; WCHCD.
Background This gene encodes a water channel protein located in the kidney collecting tubule. It belongs to the MIP/aquaporin family, some members of which are clustered together on chromosome 12q13. Mutations in this gene have been linked to autosomal dominant, and recessive forms of nephrogenic diabetes insipidus.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is AQP2 Polyclonal Antibody, Cy3 Conjugated (bs-0261R-Cy3) validated for, and at what dilution?

bs-0261R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does AQP2 Polyclonal Antibody, Cy3 Conjugated (bs-0261R-Cy3) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-0261R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise AQP2 Polyclonal Antibody, Cy3 Conjugated (bs-0261R-Cy3)?

KLH conjugated synthetic peptide derived from human AQP2

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-0261R-Cy3)?

Cy3 on bs-0261R-Cy3: excitation / emission 512,550nm/570,615nm.

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