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Neurobeachin Polyclonal Antibody, PerCP Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Fruit Fly
Overview
Catalog # bs-0268R-PerCP-100uL
Product Name Neurobeachin Polyclonal Antibody, PerCP Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Fruit Fly
Specifications
Conjugation PerCP
Host Rabbit
Source KLH conjugated synthetic peptide derived from Fruit fly Neurobeachin
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 26960
Subcellular location Cytoplasm
Synonyms A-kinase anchor protein 550; d AKAP 550; Neurobeachin protein; Rugose protein; AKAP550; NBEA_DROME.
Background Neurobeachin binds to type II regulatory subunits of protein kinase A and anchors/targets them to the membrane. It may anchor the kinase to cytoskeletal and/or organelle-associated proteins. Neurobeachin, is also a neuron-specific multidomain protein of 327 kDa with a high-affinity binding site for the type II regulatory subunit of protein kinase A. Neurobeachin is peripherally associated with pleomorphic tubulovesicular endomembranes near the trans sides of Golgi stacks and throughout the cell body and cell processes. It is also found in a subpopulation of synapses, where it is concentrated at the postsynaptic plasma membrane.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Neurobeachin Polyclonal Antibody, PerCP Conjugated (bs-0268R-PerCP) validated for, and at what dilution?

bs-0268R-PerCP is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-0268R-PerCP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Neurobeachin Polyclonal Antibody, PerCP Conjugated (bs-0268R-PerCP)?

KLH conjugated synthetic peptide derived from Fruit fly Neurobeachin

What are the excitation and emission wavelengths of the PerCP conjugate (bs-0268R-PerCP)?

PerCP on bs-0268R-PerCP: excitation / emission 482nm/677nm.