HCMV pp65 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0271R-RBITC-100uL |
| Product Name | HCMV pp65 Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from Cytomegalovirus pp65 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3077579 |
| Swiss Prot | Q6SW59 |
| Synonyms | 65 kDa phosphoprotein; pp65; 65 kDa matrix phosphoprotein; Phosphoprotein UL83; Tegument protein UL83; UL83 |
| Background | Counteracts the host antiviral immune response by preventing IRF3 from entering the nucleus once activated and phosphorylated. Participates also in the transactivation of viral major immediate-early genes by recruiting host IFI16 to their promoters. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is HCMV pp65 Polyclonal Antibody, RBITC Conjugated (bs-0271R-RBITC) validated for, and at what dilution?
bs-0271R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HCMV pp65 Polyclonal Antibody, RBITC Conjugated (bs-0271R-RBITC) react with?
Tested reactivity: Human.
How should bs-0271R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HCMV pp65 Polyclonal Antibody, RBITC Conjugated (bs-0271R-RBITC)?
KLH conjugated synthetic peptide derived from Cytomegalovirus pp65
What are the excitation and emission wavelengths of the RBITC conjugate (bs-0271R-RBITC)?
RBITC on bs-0271R-RBITC: excitation / emission 540nm/625nm.