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C Peptide Polyclonal Antibody, AbBy Fluor-488 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
Overview
Catalog # bs-0274R-BF488
Product Name C Peptide Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human
Specifications
Conjugation AbBy Fluor-488
Host Rabbit
Source KLH conjugated synthetic peptide of human C Peptide
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3630
Subcellular location Secreted
Synonyms proinsulin precursor; Hyperproinsulinemia; INS; Insulin Precursor; IRDN; Proinsulin; Propeptide; C-Peptide; INS_HUMAN.
Background C Peptide is part of the molecule of Proinsulin, that consists of three parts: C Peptide and two long strands of amino acids (called the alpha and beta chains) that later become linked together to form the insulin molecule. From every molecule of proinsulin, one molecule of insulin plus one molecule of C Peptide are produced. C peptide is released into the blood stream in equal amounts to insulin. A test of C peptide levels will show how much insulin the body is making. Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is C Peptide Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-0274R-BF488) validated for, and at what dilution?

bs-0274R-BF488 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-0274R-BF488 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise C Peptide Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-0274R-BF488)?

KLH conjugated synthetic peptide of human C Peptide

What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-0274R-BF488)?

AbBy Fluor-488 on bs-0274R-BF488: excitation / emission 499nm/519nm.