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SSB Polyclonal Antibody, RBITC Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
Overview
Catalog # bs-0276R-RBITC
Product Name SSB Polyclonal Antibody, RBITC Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Specifications
Conjugation RBITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SSB
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 6741
Subcellular location Nucleus
Synonyms SSB; Lupus La protein homolog; La ribonucleoprotein; La autoantigen homolog; Sjogren syndrome type B antigen; LA_HUMAN.
Background Lupus La protein (Sjogren syndrome type B antigen) (SS-B) (La ribonucleoprotein) (La autoantigen) plays a role in the transcription of RNA polymerase III. It is most probably a transcription termination factor. Binds to the 3' termini of virtually all nascent polymerase III transcripts. It is associated with precursor forms of RNA polymerase III transcripts including tRNA and 4.5S, 5S, 7S, and 7-2 RNAs. The phosphorylation sites are at the C-terminal part of the protein. Sera from patients with systemic lupus erythematosus(SLE) often contain that react with the normal cellular La protein as if this antigen was foreign.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is SSB Polyclonal Antibody, RBITC Conjugated (bs-0276R-RBITC) validated for, and at what dilution?

bs-0276R-RBITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does SSB Polyclonal Antibody, RBITC Conjugated (bs-0276R-RBITC) react with?

Tested reactivity: Human.

How should bs-0276R-RBITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise SSB Polyclonal Antibody, RBITC Conjugated (bs-0276R-RBITC)?

KLH conjugated synthetic peptide derived from human SSB

What are the excitation and emission wavelengths of the RBITC conjugate (bs-0276R-RBITC)?

RBITC on bs-0276R-RBITC: excitation / emission 540nm/625nm.