RRAD Polyclonal Antibody
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-0278R-100uL |
| Product Name | RRAD Polyclonal Antibody |
| Applications |
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Horse, Rabbit |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RRAD |
| Immunogen Range | 211-308/308 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 6236 |
| Subcellular location | Cell membrane |
| Synonyms | RAD; Ras-related associated with diabetes; RAD1; REM3; Cell cycle checkpoint protein Hrad1; Cell cycle checkpoint protein Rad 1 A / B; Cell cycle checkpoint protein RAD1; Checkpoint control protein HRAD1; Checkpoint control protein RAD1; DNA repair exonuclease; DNA repair exonuclease rad1; DNA repair exonuclease rad1 homolog; DNA repair exonuclease REC1; DNA repair protein RAD1; EC 3.1.11.2; Exonuclease homolog RAD1; GTP-binding protein RAD; hRAD 1; hRAD1; MGC77779; RAD 1; RAD; RAD1 homolog S. pombe; RAD1 homolog; Rad1 like DNA damage checkpoint; Rad1 like DNA damage checkpoint protein; RAD1, S. pombe, homolog of; Ras associated with diabetes; REC 1; REC1; RRAD; RAD_HUMAN. |
| Background | Human Rad1 is a component of a heterotrimeric PCNA like complex that also contains the Rad9 and Hus1 proteins. This complex is believed to be involved in cellular responses to DNA damage, possibly by associating with Rad17 and several components of the PCNA-loading heteropentamer, replication factor C. Human Rad1 exhibits significant homology to Rad1 from S. pombe, and its expression in yeast rad1 mutants has been shown to partially restore radiation resistance and G2 checkpoint activity. It has also been shown to possess exonuclease activity. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is RRAD Polyclonal Antibody (bs-0278R) validated for, and at what dilution?
bs-0278R is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-0278R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RRAD Polyclonal Antibody (bs-0278R)?
KLH conjugated synthetic peptide derived from human RRAD Immunogen range: 211-308/308.