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PLGF Polyclonal Antibody, Cy5.5 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
Overview
Catalog # bs-0281R-Cy5.5-100uL
Product Name PLGF Polyclonal Antibody, Cy5.5 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Specifications
Conjugation Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human PLGF
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5228
Swiss Prot P49763
Subcellular location Secreted
Synonyms PGFL; PLGF; PlGF-2; D12S1900; SHGC-10760; Placenta growth factor; PGF
Background Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is PLGF Polyclonal Antibody, Cy5.5 Conjugated (BS-0281R-CY5-5) validated for, and at what dilution?

BS-0281R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PLGF Polyclonal Antibody, Cy5.5 Conjugated (BS-0281R-CY5-5) react with?

Tested reactivity: Human.

How should BS-0281R-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise PLGF Polyclonal Antibody, Cy5.5 Conjugated (BS-0281R-CY5-5)?

KLH conjugated synthetic peptide derived from human PLGF

What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-0281R-CY5-5)?

Cy5.5 on BS-0281R-CY5-5: excitation / emission 675nm/694nm.