Rat IgG Isotype Control , PE-Cy5.5 Conjugated
Applications
| Overview | |
| Catalog # | bs-0293p-pe-Cy5.5 |
| Product Name | Rat IgG Isotype Control , PE-Cy5.5 Conjugated |
| Applications |
WB
FCM
|
| Specifications | |
| Conjugation | PE-Cy5.5 |
| Host | Rat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Background | Isotype controls are negative controls that are designed to measure the amount of non-specific background signal caused by the primary antibody. Isotype Control have no specificity for the target protein, yet retain all of the non-specific characteristics of an antibody. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Rat IgG Isotype Control , PE-Cy5.5 Conjugated (BS-0293P-PE-CY5-5) validated for, and at what dilution?
BS-0293P-PE-CY5-5 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should BS-0293P-PE-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What are the excitation and emission wavelengths of the PE-Cy5.5 conjugate (BS-0293P-PE-CY5-5)?
PE-Cy5.5 on BS-0293P-PE-CY5-5: excitation / emission 488nm/694nm.