Rabbit Anti-Rat IgG Antibody (H+L), Rhodamine B Isothiocyanate Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0293R-RBITC |
| Product Name | Rabbit Anti-Rat IgG Antibody (H+L), Rhodamine B Isothiocyanate Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Specifications | |
| Conjugation | Rhodamine B Isothiocyanate |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles |
| Target | |
| Background | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Rabbit Anti-Rat IgG Antibody (H+L), Rhodamine B Isothiocyanate Conjugated (bs-0293R-RBITC) validated for, and at what dilution?
bs-0293R-RBITC is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Rabbit Anti-Rat IgG Antibody (H+L), Rhodamine B Isothiocyanate Conjugated (bs-0293R-RBITC) react with?
Tested reactivity: Rat.
How should bs-0293R-RBITC be stored?
Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
What are the excitation and emission wavelengths of the Rhodamine B Isothiocyanate conjugate (bs-0293R-RBITC)?
Rhodamine B Isothiocyanate on bs-0293R-RBITC: excitation / emission 540nm/625nm.