FGL2 Polyclonal Antibody, PerCP Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0315R-PerCP |
| Product Name | FGL2 Polyclonal Antibody, PerCP Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from rat FGL2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 84586 |
| Swiss Prot | G3V7P2 |
| Subcellular location | Secreted |
| Synonyms | Fibroleukin; Cytotoxic T-lymphocyte-specic protein; Fibrinogen-like protein 2; Fibrinogen like protein 2; Prothrombinase; Fibroleukin; pT49; T49; G3V7P2_RAT, Fibrinogen-like 2. |
| Background | FGL2 is a secreted protein that is similar to the beta- and gamma-chains of fibrinogen. The carboxyl-terminus of the encoded protein consists of the fibrinogen-related domains (FRED). The encoded protein forms a tetrameric complex which is stabilized by interchain disulfide bonds. It may play a role in physiologic functions at mucosal sites. It is constitutively expressed in cytotoxic T-cells. Lack of expression in other lymphoid- and nonlymphoid-derived cell lines suggested that expression of FGL2 may be restricted to lymphocytes. FGL2 is induced via a mechanism involving IFNG and components of the IFNG signaling pathway, including STAT1 and IRF1. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is FGL2 Polyclonal Antibody, PerCP Conjugated (bs-0315R-PerCP) validated for, and at what dilution?
bs-0315R-PerCP is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does FGL2 Polyclonal Antibody, PerCP Conjugated (bs-0315R-PerCP) react with?
Tested reactivity: Rat.
How should bs-0315R-PerCP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise FGL2 Polyclonal Antibody, PerCP Conjugated (bs-0315R-PerCP)?
KLH conjugated synthetic peptide derived from rat FGL2
What are the excitation and emission wavelengths of the PerCP conjugate (bs-0315R-PerCP)?
PerCP on bs-0315R-PerCP: excitation / emission 482nm/677nm.