Mouse Anti-Human IgM Antibody (H+L), AbBy Fluor® 488 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0345M-BF488 |
| Product Name | Mouse Anti-Human IgM Antibody (H+L), AbBy Fluor® 488 Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor® 488 |
| Host | Mouse |
| Clonality | Polyclonal |
| Isotype | IgM |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles |
| Target | |
| Background | Immunoglobulin IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Mouse Anti-Human IgM Antibody (H+L), AbBy Fluor® 488 Conjugated (bs-0345M-BF488) validated for, and at what dilution?
bs-0345M-BF488 is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Mouse Anti-Human IgM Antibody (H+L), AbBy Fluor® 488 Conjugated (bs-0345M-BF488) react with?
Tested reactivity: Human.
How should bs-0345M-BF488 be stored?
Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
What are the excitation and emission wavelengths of the AbBy Fluor® 488 conjugate (bs-0345M-BF488)?
AbBy Fluor® 488 on bs-0345M-BF488: excitation / emission 499nm/519nm.