Mouse Anti-Rat IgM Antibody (H+L)
Applications
Reactivity
| Overview | |
| Catalog # | bs-0346M |
| Product Name | Mouse Anti-Rat IgM Antibody (H+L) |
| Applications |
WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Clonality | Polyclonal |
| Isotype | IgM |
| Concentration | Determined by end user |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Background | Immunoglobulin IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| FCM | 1:20-100 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Mouse Anti-Rat IgM Antibody (H+L) (bs-0346M) validated for, and at what dilution?
bs-0346M is validated for WB (1:300-5000); ELISA (1:500-1000); FCM (1:20-100); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Mouse Anti-Rat IgM Antibody (H+L) (bs-0346M) react with?
Tested reactivity: Rat.
How should bs-0346M be stored?
Store at -20°C for 12 months. Storage buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.