Mouse Anti-Rat IgM Antibody (H+L), Biotin Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0346M-Biotin |
| Product Name | Mouse Anti-Rat IgM Antibody (H+L), Biotin Conjugated |
| Applications |
WB
IHC-P
IHC-F
|
| Reactivity | Rat |
| Specifications | |
| Conjugation | Biotin |
| Host | Mouse |
| Clonality | Polyclonal |
| Isotype | IgM |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Background | Immunoglobulin IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is Mouse Anti-Rat IgM Antibody (H+L), Biotin Conjugated (bs-0346M-Biotin) validated for, and at what dilution?
bs-0346M-Biotin is validated for WB (1:300-5000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Mouse Anti-Rat IgM Antibody (H+L), Biotin Conjugated (bs-0346M-Biotin) react with?
Tested reactivity: Rat.
How should bs-0346M-Biotin be stored?
Store at -20°C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.