Histone H3 Polyclonal Antibody, HRP Conjugated

Applications

  • WB
  • IHC-P
  • IHC-F

Reactivity

  • Human
  • Mouse
  • Rat
  • Others

Predicted Reactivity

  • Cow
  • Pig
  • Rabbit
  • Drosophila
Overview
Catalog # bs-0349R-HRP
Product Name Histone H3 Polyclonal Antibody, HRP Conjugated
Applications WB, IHC-P, IHC-F
Reactivity Human, Mouse, Rat, Others
Predicted Reactivity Cow, Pig, Rabbit, Drosophila
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Histone H3
Immunogen Range 71-136/136
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles
Target
Gene ID 3020
Swiss Prot P84243
Synonyms Histone H3; H3.1; HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; H3FA; H3FL; H3FC; H3FB; H3FD; H3FI; H3FH; H3FK; H3FF; H3FJ; H3.2; HIST2H3A; HIST2H3C; HIST2H3D; H3F2; H3FM; H3.3; H3F3A; H3F3B; H3.3A; H3.3B; H3F3; PP781; H3.3C; H3F3C; H3.5; H3.1t; HIST3H3; H3FT
Background Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Application Dilution
WB 1:300-1000
IHC-P 1:200-400
IHC-F 1:100-500