Rabbit Anti-Mouse IgM Antibody (H+L), Phycoerythrin Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0368R-PE |
| Product Name | Rabbit Anti-Mouse IgM Antibody (H+L), Phycoerythrin Conjugated |
| Applications |
FCM
|
| Reactivity | Mouse |
| Specifications | |
| Conjugation | Phycoerythrin |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgM |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles |
| Target | |
| Background | Immunoglobulin IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. |
| Application Dilution | |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Rabbit Anti-Mouse IgM Antibody (H+L), Phycoerythrin Conjugated (bs-0368R-PE) validated for, and at what dilution?
bs-0368R-PE is validated for FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Rabbit Anti-Mouse IgM Antibody (H+L), Phycoerythrin Conjugated (bs-0368R-PE) react with?
Tested reactivity: Mouse.
How should bs-0368R-PE be stored?
Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
What are the excitation and emission wavelengths of the Phycoerythrin conjugate (bs-0368R-PE)?
Phycoerythrin on bs-0368R-PE: excitation / emission 496,564nm/578nm.