Mouse Anti-Rabbit IgM Antibody (H+L), Cyanine 5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0369M-Cy5.5 |
| Product Name | Mouse Anti-Rabbit IgM Antibody (H+L), Cyanine 5.5 Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rabbit |
| Specifications | |
| Conjugation | Cyanine 5.5 |
| Host | Mouse |
| Clonality | Polyclonal |
| Isotype | IgM |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles |
| Target | |
| Background | Immunoglobulin IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Mouse Anti-Rabbit IgM Antibody (H+L), Cyanine 5.5 Conjugated (BS-0369M-CY5-5) validated for, and at what dilution?
BS-0369M-CY5-5 is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Mouse Anti-Rabbit IgM Antibody (H+L), Cyanine 5.5 Conjugated (BS-0369M-CY5-5) react with?
Tested reactivity: Rabbit.
How should BS-0369M-CY5-5 be stored?
Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
What are the excitation and emission wavelengths of the Cyanine 5.5 conjugate (BS-0369M-CY5-5)?
Cyanine 5.5 on BS-0369M-CY5-5: excitation / emission 675nm/694nm.