Runx3 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0378R-Cy3 |
| Product Name | Runx3 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat, Bovine, Dog, Chicken |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Runx3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 864 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | RUNX 3; RUNX-3; Runt-related transcription factor 3; Core-binding factor, alpha 3 subunit; CBF-alpha 3; Acute myeloid leukemia 2 protein; OncogeneAML-2; Polyomavirus enhancer-binding protein 2 alpha C subunit; PEBP2-alpha C; PEA2-alpha C; SL3-3 enhancer factor 1 alpha C subunit; SL3/AKV core-binding factor alpha C subunit; Core-binding factor, alpha 3 subunit; CBF-alpha 3; Acute myeloid leukemia 2 protein; Oncogene AML-2; Polyomavirus enhancer-binding protein 2 alpha C subunit; PEBP2-alpha C; PEA2-alpha C; SL3-3 enhancer factor 1 alpha C subunit; RUNX3_HUMAN; SL3/AKV core-binding factor alpha C subunit. |
| Background | RUNX3 binds to the core site of murine Leukemia virus, the core sequences in the enhancer of the polyomavirus, and also to the enhancers of the T-cell receptor genes. May be involved in the control of cellular proliferation and/or differentiation (By similarity). Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain.DNA-binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1, Subcellular location in Nucleus. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | IHC-P1:50-200 |
Frequently asked questions
Which applications is Runx3 Polyclonal Antibody, Cy3 Conjugated (bs-0378R-Cy3) validated for, and at what dilution?
bs-0378R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Runx3 Polyclonal Antibody, Cy3 Conjugated (bs-0378R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat, Bovine, Dog, Chicken.
How should bs-0378R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Runx3 Polyclonal Antibody, Cy3 Conjugated (bs-0378R-Cy3)?
KLH conjugated synthetic peptide derived from human Runx3
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-0378R-Cy3)?
Cy3 on bs-0378R-Cy3: excitation / emission 512,550nm/570,615nm.