MBP Polyclonal Antibody, PE Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0380r-pe-100l |
| Product Name | MBP Polyclonal Antibody, PE Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from Guinea Pig MBP |
| Immunogen Range | 69-85/167 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Nucleus, Cell membrane |
| Synonyms | MBP_CAVPO;Myelin Basic Protein; Myelin basic protien; GDB; Golli MBP; Hemopoietic MBP; HMBPR; HUGO; MBP; MGC99675; MLD; Myelin A1 Protein; Myelin Deficient; Myelin Membrane Encephalitogenic Protein; SHI; Shiverer; SP; MBP TAG; MBP-TAG |
| Background | Oligodendrocyte Marker The classic group of Myelin basic protein (MBP) isoforms (isoforms 4 to 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non classic group of MBP isoforms (isoforms 1 to 3/Golli MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T cells and neural cells. Differential splicing events combined to optional posttranslational modifications give a wide spectrum of isomers, each of them having maybe a specialized function. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF | 1:1000-5000 |
Frequently asked questions
Which applications is MBP Polyclonal Antibody, PE Conjugated (bs-0380R-PE) validated for, and at what dilution?
bs-0380R-PE is validated for WB (1:500-2000); IF (1:1000-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MBP Polyclonal Antibody, PE Conjugated (bs-0380R-PE) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Horse, Rabbit.
How should bs-0380R-PE be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MBP Polyclonal Antibody, PE Conjugated (bs-0380R-PE)?
KLH conjugated synthetic peptide derived from Guinea Pig MBP Immunogen range: 69-85/167.
What are the excitation and emission wavelengths of the PE conjugate (bs-0380R-PE)?
PE on bs-0380R-PE: excitation / emission 496,564nm/578nm.