CRF Polyclonal Antibody

Formalin-fixed and paraffin embedded: rat kidney tissue labeled with Anti-CRF/CRH Polyclonal Antibody, Unconjugated (bs-0382R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded: rat kidney tissue labeled with Anti-CRF/CRH Polyclonal Antibody, Unconjugated (bs-0382R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded: rat kidney tissue labeled with Anti-CRF/CRH Polyclonal Antibody (bs-0382R), Unconjugated at 1:200, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, PE conjugated (bs-0295G-PE) at 1:200 for 40 minutes at 37°C DAPI(5ug/ml, blue) was used to stain the cell nuclei

Formalin-fixed and paraffin embedded: rat kidney tissue labeled with Anti-CRF/CRH Polyclonal Antibody (bs-0382R), Unconjugated at 1:200, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, PE conjugated (bs-0295G-PE) at 1:200 for 40 minutes at 37°C DAPI(5ug/ml, blue) was used to stain the cell nuclei

Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with CRF Polyclonal Antibody, Unconjugated (bs-0382R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.

Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with CRF Polyclonal Antibody, Unconjugated (bs-0382R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.

Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with CRF Polyclonal Antibody (bs-0382R) at 1:400 overnight at 4°C, followed by a FITC conjugated secondary and DAPI staining.

Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with CRF Polyclonal Antibody (bs-0382R) at 1:400 overnight at 4°C, followed by a FITC conjugated secondary and DAPI staining.

Applications

IHC-P
IF(IHC-P)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-0382R
Product Name	CRF Polyclonal Antibody
Applications	IHC-P, IF(IHC-P)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CRF
Immunogen Range	148-196/196
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage	Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
Target
Gene ID	1392
Swiss Prot	P06850
Subcellular location	Secreted
Synonyms	CRF; Corticoliberin; Corticotropin-releasing factor; Corticotropin-releasing hormone; CRH
Background	This hormone from hypothalamus regulates the release of corticotropin from pituitary gland.
Application Dilution
IHC-P	1:200-400
IF(IHC-P)	1:50-200