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    CRF Polyclonal Antibody

    Formalin-fixed and paraffin embedded: rat kidney tissue labeled with Anti-CRF/CRH Polyclonal Antibody, Unconjugated (bs-0382R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
    Formalin-fixed and paraffin embedded: rat kidney tissue labeled with Anti-CRF/CRH Polyclonal Antibody (bs-0382R), Unconjugated at 1:200, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, PE conjugated (bs-0295G-PE) at 1:200 for 40 minutes at 37°C DAPI(5ug/ml, blue) was used to stain the cell nuclei
    Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with CRF Polyclonal Antibody, Unconjugated (bs-0382R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
    Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with CRF Polyclonal Antibody (bs-0382R) at 1:400 overnight at 4°C, followed by a FITC conjugated secondary and DAPI staining.
    Description:
    Applications:
    • IHC-P
    • IF(IHC-P)
    Overview
    Catalog # bs-0382R
    Product Name CRF Polyclonal Antibody
    Applications IHC-P, IF(IHC-P)
    Reactivity Human, Mouse, Rat
    Specifications
    Conjugation Unconjugated
    Host Rabbit
    Source KLH conjugated synthetic peptide derived from human CRF
    Immunogen Range 148-196/196
    Clonality Polyclonal
    Isotype IgG
    Concentration 1ug/ul
    Purification Purified by Protein A.
    Storage Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
    Target
    Gene ID 1392
    Swiss Prot P06850
    Subcellular location Secreted
    Synonyms CRF; Corticoliberin; Corticotropin-releasing factor; Corticotropin-releasing hormone; CRH
    Background This hormone from hypothalamus regulates the release of corticotropin from pituitary gland.
    Applications
    Application Dilution
    IHC-P 1:100-500
    IF(IHC-P) 1:50-200