HOXc8 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human Cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HOXc8 Polyclonal Antibody, Unconjugated (bs-0394R) at 1:500 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human Cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with HOXc8 Polyclonal Antibody, Unconjugated (bs-0394R) at 1:500 overnight at 4\u00b0C, DAB staining.

Rat liver lysates probed with HOXc8 Polyclonal Antibody, Unconjugated (bs-0394R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Applications

WB
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Rat

Predicted Reactivity

Mouse
Cow
Pig
Horse

Overview
Catalog #	bs-0394R
Product Name	HOXc8 Polyclonal Antibody
Applications	WB, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Rat
Predicted Reactivity	Mouse, Cow, Pig, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human HOXc8
Immunogen Range	51-130/242
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3224
Subcellular location	Nucleus
Synonyms	Hox-3.1; Homeo box 3A; Homeo box 8; Homeobox 3A; Homeobox C8; Homeobox protein Hox C8; Homeobox protein Hox-C8; Homeobox protein HoxC8; Homeobox protein R4;Homolog of mouse Hox 3.1; HOX 3; Hox 3.1 mouse homolog of; HOX 3A; HOX C8; Hox-3.1, MOUSE, HOMOLOG OF; Hox-3A; HOX3; HOX3A; HOXC 8; Hoxc-8; M31.
Background	This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, which are located on different chromosomes and consist of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXC genes located in a cluster on chromosome 12. The product of this gene may play a role in the regulation of cartilage differentiation. It could also be involved in chondrodysplasias or other cartilage disorders.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200